A novel flavivirus in the soybean cyst nematode

Engineered Soybean Cyst Nematode Resistance

Distribution of soybean cyst nematode in nebraska.

A survey of 552 soybean fields in 20 counties in Nebraska in 1986-88 revealed 35 fields infested with the soybean cyst nematode (SCN), Heterodera glycines. Identification was confirmed with a greenhouse bioassay, using 'Lee 74' soybean, and by the application of a DNA hybridization probe derived from SCN mitochondrial DNA. Most of the SCN-infested fields were located on the Missouri River flood...

The mitochondrial genome of the soybean cyst nematode, Heterodera glycines.

We sequenced the entire coding region of the mitochondrial genome of Heterodera glycines. The sequence obtained comprised 14.9 kb, with PCR evidence indicating that the entire genome comprised a single, circular molecule of approximately 21-22 kb. The genome is the most T-rich nematode mitochondrial genome reported to date, with T representing over half of all nucleotides on the coding strand. ...

Survey of soybean cyst nematode races in tennessee.

Cordell soybean is a newly released cultivar resistant to Heterodera glycines (SCN) races 3 and 5. The hectarage infested with these races and with other races that can parasitize Cordell in Tennessee is not known. A survey of races in Tennessee soybean fields with sampling weighted by soybean hectarage on a county basis was conducted in 1988. The SCN race plus female development on Bedford and...

Molecular characterisation of two novel 17β-hydroxysteroid dehydrogenase genes from the soybean cyst nematode Heterodera glycines

Heterodera glycines Hg-hsd-1 and Hg-hsd-2 are two homologues of let-767, a short-chain dehydrogenase gene from the free-living nematode Caenorhabditis elegans. Both genes share similarity to 17β-hydroxysteroid dehydrogenases (17β-HSDs), enzymes known to be involved in the production of steroid hormones in mammals. Hg-hsd-1 and Hg-hsd-2 encode distinct protein sequences that share 51% amino acid...